Abstract
1939 was a problematic year for Europe and the World, as it was the beginning of World War 2. However, presumably while Hitler tanks were crossing the Polish border, another historical event (that would also have changed the World as we knew it) was taking place in a small marine biology laboratory in Plymouth. That year, Alan Hodgkin and Andrew Huxley were sticking electrodes in the giant axon of a squid. They observed, for the first time, that neurons plasma membrane possesses an electrical resting potential at around -65 mV; that if depolarized to a certain threshold value, it could result in action potentials; and that action potentials rely on voltage-dependent generation of inward Na+ and outward K+ currents. Particularly, this latter part was shown by using a technique that they called voltage-clamp. Now, after almost 80 years, we know that single-cell electrophysiological properties are central for brain function. Indeed, modern neuroscience aims to bridge single cell electrophysiology, neural network electrical oscillations and behaviour in healthy and mentally ill individuals. In our laboratory, we focus on characterizing, with a multidisciplinary approach, the functional, electrophysiological correlates of cognitive functions, especially memory, with a particular focus on Alzheimer's disease and dementia. To this end, I spent the last few years carrying out single-neuron, patch-clamp electrophysiological recordings in acute brain slices from mice carrying genes associated to dementia; lately, I also started to carry out the same experiments in 2D and 3D neural networks, obtained by culturing stem cell-derived neurons of human and animal origin. In this talk, I will illustrate that neurons from dementia models show hyperexcitability, as a possible correlate of increased risk of seizure observed in patients with dementia. In addition, I will illustrate my observations on the level of electrophysiological maturation of stem-cell derived neurons, cultured in different conditions.
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University of Reading Malaysia
Persiaran Graduan, Kota Ilmu, EduCity, 79200 Iskandar Puteri, Johor, Malaysia.
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